How to Get a Urine Stain Out of Grout

Whether you have a little boy with bad aim or incontinent pets, urine stains are tough to remove from grouted tiled floors and walls. Urine soaks into the porous surface of grout and textured tiles to cause ugly discoloration and odors. Use enzyme cleaners to remove the urine's odor causing bacteria, grout cleaner to retain the original grout color and then seal the tiled surface to prevent future urine stains.

Step 1

Spray a commercial enzyme cleaner, like Nature's Miracle or Kids & Pets, on the stained grout areas to remove the urine bacteria and odors. Allow the cleaner to stand and soak into the porous grout surface. Scrub with a toothbrush or rough-textured sponge.

Step 2

If the urine stains still appear in color after removing the odors and bacteria, use a commercial grout cleaner, like Finazzle, to return the grout color. Spray the acid-based grout cleaner on the stained area, let stand and scrub off.

Step 3

Use a hand-held blacklight to locate areas of urine-causing odors without visible stains. Turn off bathroom lights, pass a blacklight around the floor and watch for any spots that glow. Mark these areas. Clean the invisible stains with the enzyme urine remover to remove remaining urine odors completely.

Step 4

After cleaning the tiled bathroom floor, brush or spray grout sealant over entire tile surface. Allow it to dry overnight. Apply a 2nd coat of sealant to prevent urine from reaching the porous grout surface and staining. Re-seal the bathroom floor each year.

Step 5

Use commercial tile and grout cleaner each week to avoid letting urine stains sit in the grout. Urine is easiest to remove within a few days of the accident. Spray the commercial cleaner over the surface, allow it to stand a few minutes and then scrub with a toothbrush or textured brush.

Step 6

Avoid noticeable urine stains by choosing large bathroom tiles at least 6 inches wide. Use narrow grout seams to create a smaller grout surface area and less chance of staining. You can use a slightly darker grout instead of beige or white to camouflage urine stains around the base of the toilet.