How to Grow Muscadine Grapes From Seed

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Things You'll Need

  • Muscadine grape seeds

  • Peat moss

  • 4-inch pots

  • Sterile potting soil

  • Spray bottle

  • 6-inch pots

  • Soil testing kit

  • Garden fork

  • Lime or peat moss

  • Soaker hose

  • Flexible cording

  • Stakes

  • Trellises

  • Mulch

  • Fertilizer

  • Grow lights

  • Plant heating pad

Muscadine grapes grow in small clusters.

Native to Florida and the southern portion of the U.S., muscadine grapes grow in small clusters. Most grape farmers use muscadine grapes in wines, jams, jellies and juices, but the fruit also offers a pleasing taste when eaten directly from the vine. Muscadine grapes prefer to grow in sunny, well-drained soils of USDA hardiness zones 6 through 9. Within one of these hardiness zones you can plant muscadine grapes successfully in your background landscape.


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Seed Germination

Step 1

Remove muscadine grape seeds from the ripe fruit. Remove any attached pulp. Rinse with lukewarm water. Lay the seeds on a paper plate to dry, undisturbed for 24 hours.

Step 2

Place damp peat moss into a plastic sandwich until it is half full. Drop the muscadine grape seeds into the baggie and seal it. Place the baggie in the refrigerator for three months.

Step 3

Pour sterile potting soil into 4-inch pots until they are three-fourths full. Remove the muscadine grape seeds from the refrigerator after the three-month stratification period. Push two to three seeds ¼ of an inch into the soil of each pot.


Step 4

Mist the soil with lukewarm water. Set the pots on a tray. Place the tray in an area that receives bright, indirect light. Maintain a temperature of approximately 75 degrees F during the germination process, which usually begins within two to three weeks.

Step 5

Maintain bright light and moist soil conditions as the muscadine grape seedlings continue to grow. Transplant the seedlings into 6-inch pots when they outgrow the 4-inch pots. Transplant the seedlings outdoors after a full year of growth.

Transplanting Outdoors

Step 1

Dig holes large enough to accommodate the root balls of the muscadine grape seedlings. The leaves of the plants should remain above the soil line when positioned in the holes. Space the holes 20 feet apart.


Step 2

Keep the soil moist at a 1-inch depth throughout the growing season. After the first year of growth, muscadine grapes will only require supplemental watering during drought.

Step 3

Secure each of the muscadine grape plants with flexible cording attached to a stake. Set up a trellis behind each of the stakes to guide the muscadine grape plants upward as they grow.


Prepare the soil before spring planting outdoors. Muscadine grapes prefer soil with a pH between 6.0 and 6.5. Purchase a soil testing kit from a garden center if you do not know the pH of your soil. Apply mulch around the muscadine grape plants. A 3-inch layer of straw or bark chips will discourage weed growth and help with water retention. Feed the muscadine grapes a diet of 10-10-10 fertilizer. Apply the fertilizer according to the label instructions. If you cannot provide the muscadine seeds with bright, indirect sunlight during the germination period, setup a grow light. Use a plant heating pad to maintain the required heat, if necessary. Allow the muscadine grape vines to die off and enter dormancy at the end of each fall season. Remove the previous year’s growth with a pair of pruning shears to encourage new growth in the spring. Follow the procedure each winter for the first four growing seasons.

Begin harvesting fruit in the third growing season.


Do not over-water the muscadine grape plants. Over-watering can lead to root rot, a disease that can eventually kill the plants. Check the soil for moisture by pressing your fingers down 1 inch into the soil. If the soil feels moist, skip the watering.


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