How to Remove Urine Odor From Wood Subflooring

Although pets are wonderful to have, they sometimes can cause problems in the home. Urinating on flooring is not uncommon among household pets such as dogs and cats. Sometimes, the urine seeps through the carpet and into the subflooring. It may be necessary remove the carpet and clean the subflooring to eliminate all of the urine odor. Wood is a common type of subflooring and must be thoroughly cleaned to remove urine odors.

Step 1

Soak up as much of the urine as possible using old rags and allow the area to dry.

Step 2

Mix equal parts of white vinegar and warm water into an spray bottle. Shake the bottle to thoroughly combine the ingredients.

Step 3

Spray the vinegar-water solution onto the affected area of subflooring. Rub it into the flooring using a rag.

Step 4

Allow the vinegar solution to dry completely. Wipe down the area with a rag and warm water.

Step 5

Combine 15 oz of peroxide, 2 tbsp of baking soda and two squirts of hand soap into a plastic container if the urine odor persists. Mix the ingredients with a spoon.

Step 6

Pour the solution directly onto the affected area. Allow the solution to sit for a few minutes, then rinse clean with a rag and warm water.

Step 7

Clean the area with mouthwash to ensure that the odor is gone. Mix two parts of water to one part of mouthwash into an empty spray bottle. Spray it onto the subfloor, scrub it in with a rag and let it sit for at least two hours. Wipe clean with a wet rag.

Step 8

Combine chlorine bleach and water into a spray bottle for an alternative urine odor-removal method and to disinfect the area. Mix eight parts of water to one part of bleach in the bottle. Spray the urine-stained area of the subflooring with the bleach mixture. Do not rinse or wipe away the bleach.