Cats provide companionship and love, all wrapped up in a cute little ball of fur. Unfortunately, cat urine can leave an odor that lasts for weeks and even months after the initial accidents. A variety of problems -- both emotional and physical -- will cause your cat to urinate outside its litter box, and a trip to a veterinarian may be in order. No matter what the reason, you can clean the cat-urine odor from tile and grout.
Absorb fresh urine with several paper towels folded in half. Discard the soiled paper towels.
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Pour undiluted white vinegar into a spray bottle. Mist the tiles and grout with the vinegar. Rub with a damp cloth. Vinegar is an all-natural cleaner and deodorizer that neutralizes the cat urine and removes the odor.
Wipe the vinegar from the floor with a dry cloth. Mist the tiles and grout with the vinegar again. Let the vinegar air-dry on the floor.
Fill a bucket with 1 gallon cool water and 1 cup oxygen bleach. Dip an old toothbrush into the mixture and scrub the grout vigorously. The oxygen bleach will help brighten the grout and remove any urine stains.
Saturate a sponge in the mixture and wring out the excess liquid. Wipe the tiles and grout with the sponge. Dampen a clean cloth with cool water and rinse the mixture from the tiles. Wipe the tiles and grout clean with a towel.