Urine, whether from humans or pets, contains urea and odor-causing bacteria that can cause discoloration and odor in your wood floors. When an accident has occurred on wood flooring, respond as quickly as possible before the stain causes damage. While it's easiest to remove a fresh urine stain, you can even remove older stains with a little more effort. Either way, removing the urine from the wood floors is imperative to avoid having pets return to the area to eliminate as well as lingering smells.
Lay paper towels over the stain to soak up any urine sitting on the surface of the wood floor. Discard soiled paper towels and continue to lay down fresh ones until you're no longer able to pick up urine.
Cover the site of the urine stain with baking soda and allow it to sit for an hour. The baking soda absorbs any fresh urine soaked into the wood while deodorizing the wood.
Vacuum the baking soda from your wood floor. Mix a capful of mild, oil-based soap in a gallon of warm water and wash the surface of the floor with a clean cloth dampened with the solution. Rinse the floor with a plain, damp cloth and then dry with another cloth.
Allow the wood floor to dry overnight so you can see if the urine caused any bleaching or discoloration to the wood. If bleaching or discoloration exists, it indicates the urine has soaked into the fibers of the wood.
Wet a clean cloth with hydrogen peroxide and lay it over the site of the urine stain. Place a piece of plastic wrap on top of the peroxide-soaked cloth. Leave the two in place for a couple hours or overnight.
Take the plastic wrap and cloth off the floor and wash it with your mild oil-based soap solution. Rinse and dry the floor and allow it to air dry completely.
Sand the wood floor with 80-grit sandpaper to remove any remaining discoloration and prepare the floor for wood floor finish.
Touch up the wood floor with a matching wood stain. Apply the stain in the direction of the wood grain. Allow it to dry completely before walking on the floor.